Early administration of DAF improves BOP-induced neurodegeneration. Coronal frozen sections of 20-μm thickness were stained with Fluoro-Jade B as described in the Methods. Representative Fluoro-Jade B staining in frontal grey matter ( a ) and hippocampus (DG) ( b ) following BOP. n = 8 for control and experimental groups. Original magnification of × 200. Scale bar, 200 μm.