Early treatment of DAF attenuates brain injury of rats exposed to 120 kPa BOP. a and c: Representative photomicrographs of coronal paraffin sections stained with H&E of control, BOP-3 h, BOP-24 h and BOP-48 h untreated and treated with rhDAF (50 μg/kg) 30 min post blast exposure. a: brain frontal cortical grey matter and c: hippocampal dentate gyrus, DG. Original magnification of × 400. Scale bar, 100 μm. b and d: Mean brain injury scores, n = 8 for control, 3 and 24 h experimental groups, n = 5 for 48 h experimental groups. Injury scores calculated using the criteria as described in the Methods. Graphs expressed as mean ± SEM, and compared using one-way ANOVA followed by Bonferroni test.