Fig. 8
From: LINC complex alterations are a key feature of sporadic and familial ALS/FTD
SUN1 nuclear loss is a main contributor to cellular morphological alterations. (a) Representative images of cortical neurons categorized based on the presence of nuclear (Nuc.) or cytoplasmic (cyto.) TDP-43 (green) and normal (Norm.) or abnormal (Abn.) SUN1 staining (red). MAP2 (grays) was used as a neuronal marker, while DAPI (blue) labeled the cell nucleus. Scale bar: 10 μm. (b) Quantification of the relative nuclear to cytoplasmic area shows that neurons with abnormal SUN1 distribution have significantly smaller nuclei compared to all other groups (one-way ANOVA with Tuckey post hoc test, n = 22, 21,29, 20, ****p < 0.0001). (c) Quantification of the absolute nuclear area of cortical neurons shows that all ALS neurons have smaller nuclei compared to healthy controls. However, loss of SUN1 from the NE (categorized as abnormal) further impacts nuclear size, regardless of TDP-43 localization (one-way ANOVA with Tuckey post hoc test, n = 22, 21,31, 22, *p < 0.05, **p < 0.01, ****p < 0.0001)