Table 4 Antibodies used for IHC and deep spatial profiling
Antibody | Target | Host | Dilution | Company |
|---|---|---|---|---|
IHC | ||||
Iba1 | Iba1 | rabbit | 1:8000 | Abcam |
P2RY12 | P2RY12 | rabbit | 1:1000 | Merck/Millipore Sigma (HPA014518) |
Ferritin | Ferritin | rabbit | 1:1000 | Merck/Millipore Sigma (F6136-1MG) |
CD68 | CD68 | rabbit | 1:2000 | ThermoFisher SCIENTIFIC (PA578996) |
Ab5 | Aβ plaques | mouse | 1:1000 | [41] |
AT8 | Phospho-tau | mouse | 1:5000 | Invitrogen (MN1020) |
94-3A10 | α-Synuclein | mouse | 1:10,000 | [73] |
DSP | ||||
GeoMx® Parkinson’s Morphology Kit | MAP-2 & α-Synuclein | 1:40 | NanoString® (121,300,307) | |
Parkinson’s pathology panel | 10 targets for human PD pathology | 1:25 | NanoString® (121,300,110) | |
Glial cell subtyping | 10 targets for human glial cell subtyping | 1:25 | NanoString® (121,300,116) | |
Neuronal cell profiling | 20 targets for human neural cell profiling | 1:25 | NanoString® (121,300,108) | |
Beta-Amyloid (D54D2) | Aβ plaques | 1:40 | Cell signaling technology (35363S) | |
Alzheimer’s pathology panel | 10 targets for human AD pathology | 1:25 | NanoString® (121,300,109) | |
Alzheimer’s extended pathology panel | 10 targets for human AD pathology | 1:25 | NanoString® (121,300,114) |