Fig. 6From: Gene-expression profiling of individuals resilient to Alzheimer's disease reveals higher expression of genes related to metallothionein and mitochondrial processes and no changes in the unfolded protein responseValidation of the unfolded protein response with IHC. A Representative images of HSP70 levels in the different groups. B Quantification of HSP70 immunoreactivity (HSP70-ir), which is significantly increased in the AD compared to the resilient and control cases (Kruskal–Wallis H = 10.81, p < 0.0045, AD versus control; p = 0.0145, resilient versus control; p > 0.999, resilient versus AD; p = 0.0146). C Representative images of XBP1s in the different groups. D Quantification of XBP1s immunoreactivity (XBP1s-ir), which is significantly increased in the AD compared to the resilient cases (Kruskal–Wallis H = 8.73, p < 0.0127, AD versus control; p > 0.999, resilient versus control; p = 0.0971; resilient versus AD; p = 0.0116). E and F Fluorescent IHC stains show that HSP70 (green) is present in GFAP-positive astrocytes (red), NeuN-positive neurons (red) and in Iba1-postive astrocytes (white). Double-labeled astrocytes are denoted with an arrow and neurons with an asterisk, nuclei are stained with DAPI (blue). G and H Fluorescent IHC images show XBP1s (green) is present in NeuN-positive neurons (red), GFAP-positive astrocytes (red) and Iba1-positive microglia (white). Double-labeled astrocytes are denoted with an arrow and neurons with an asterisk, nuclei are stained with DAPI (blue). Scale bars in all panels are 50 µm, p < 0.05: *Back to article page