Fig. 3

Macrophages in the perivascular space of Sturge-Weber brain. a Staining for MRC1 (green), UEAI (endothelium, red) and counterstaining for DAPI (blue) in SWS brain sections (top), non-SWS brain sections (middle), and inflamed brain sections (bottom). Quantification of MRC1⁺ cells (right). b Co-staining for CD68 (yellow), MRC1 (grey), UEAI (red) and counterstaining for DAPI (blue) in the SWS brain (top), non-SWS brain (middle), and inflamed brain (bottom). Quantification of co-labeled MRC1⁺ and CD68⁺ cells (right) c Co-staining of lymphatic vessel endothelial hyaluronan receptor 1 (LYVE1, yellow), MRC1 (grey), UEAI (red) and counterstaining for DAPI (blue) in the SWS brain (top), non-SWS brain (middle), and inflamed brain (bottom). Quantification of co-labeled MRC1⁺ and LYVE1⁺ cells (right). The p-values were calculated by One-way ANOVA test with Dunn’s multiple comparison test. d Triple staining of MRC1⁺, CD68⁺, and LYVE1⁺ in the SWS brain. Quantification (right) shows more than 95% of cells are triple positive for MRC1, CD68, and LYVE1. See Table 1 for information on brain specimens. SWS brain (n = 4), non-SWS brain (n = 2) and inflamed brain (n = 1) specimens. All scale bars are 50 µm