Fig. 1

Characterization of blood vessels in the Sturge-Weber brain. a Endothelial staining with Ulex europaeus agglutinin-I (UEAI, red) and nuclei counterstaining with DAPI (blue) (top), scale bar = 500 µm; Hematoxylin and Eosin (H&E) (middle), scale bar = 50 mm; and Martius Scarlet Blue (bottom) staining, scale bar = 500 µm, fibrin (red), collagen (blue), erythrocytes (yellow), and nuclei (blue/black) in SWS brain, non-SWS brain, and inflamed brain. b Quantification of the vessel area (µm²) and circumference (µm) in the SWS brain (n = 4), non-SWS brain (n = 2) and inflamed brain (n = 1) specimens. The p-values were calculated by One-way ANOVA followed by Dunn’s multiple comparison test