Fig. 6

Hypoxia remodels the cerebral organoid microenvironment and increases the secretion of pro-tumorigenic ligands. a UMAP of hypoxic culture CO cohort colored by oxygen condition. b UMAP of hypoxic culture CO cohort colored by cell type. c Cell type proportions identified in each CO sample. d Dot plot of cluster marker gene expression used for cell type classification. e Scaled cell stress signature scores, single cells grouped sample. f Violin plots of candidate pro-tumorigenic ligand expression in hypoxic culture CO cohort. g Violin plot of glioma TAA signature scores in hypoxic vs control astrocytes. h Effect of recombinant acidic FGF1 on 320_GSC growth. i Proportion of H1-ESC and 320-GSC specific SNP fingerprints detected in control 5-month-old ltGLICO and 5-month-old ltGLICO treated for 6 weeks with 50uM Buthionine sulfoximine (BSO)