Fig. 2
From: Chronic hypoxia remodels the tumor microenvironment to support glioma stem cell growth
The organoid component of ltGLICOs recapitulates human neurodevelopment for up to 12 months. a UMAP of the CO cells from the ageing ltGLICO cohort colored by cell subtype clusters. b UMAP of the CO cells from the ageing ltGLICO cohort colored by cell type. c Dot plot of cluster marker gene expression used for cell type classification. Top; Cluster cell proportion by ltGLICO sample age. d Multiplex immunofluorescence staining of a representative neural rosette from a 1-month-old CO. DAPI; blue, NESTIN; cyan, EOMES; orange, NEUROD2; green. Scale bar = 100 µm. e Cell type and cell cycle stage proportions identified in each ltGLICO sample. f UMAP and Violin plot showing glioma tumor associated astrocytes (TAA) gene signature scores in single cells