Fig. 6

Effect of PLA2R1 knockdown causes metabolic reprogramming via MAPK pathway. a Graph illustrating the Seahorse Cell Energy Phenotype of PLA2R1 KD cells and controls (NT and LacZ) and depicting alterations in OCR and ECAR levels both prior to (baseline) and following (maximal) the introduction of mitochondrial stressors. b and c Barplot showing differences in baseline and maximal OCR and ECAR (Error bars with SD and p-values t-test comparison between sg1 compared against LacZ). d Immunoblots for phosphorylated and total MEK1/2, ERK1/2 and c-Myc post PLA2R1 downregulation. e Proposed model showing PLA2R1 is involved in MAPK/ERK signalling pathway. PLA2R1 knockdown in chordoma cells causes metabolic reprogramming by reducing OXPHOS and glycolysis, hence inhibiting cell growth and proliferation mediated by the MAPK pathway