Fig. 5
Effect of CRISPR/Cas9 knockdown on chordoma cell growth and survival. a Expression of PLA2R1 in UM-Chor1 following CRISPR-mediated knockdown of PLA2R1. b Digital image of colony formation assay after PLA2R1 knockdown. c Images of spheroid growth showing effect of PLA2R1 KD for a period of 28 days (Scale bar = 500 μM). Boxplots with jitters showing spheroid size (p-values calculated with t-test test between sg1 and sg2 compared against LacZ). e PLA2R1 KD (sg1 and sg2) and controls cells (NT and LacZ) were subcutaneously injected into NSG mice (N = 4). f Growth curve of xenograft tumor volume (average values with standard deviation) over a period of 120 days showing PLA2R1 KD negatively impacts in vivo tumor growth compared to controls. g Digital image of tumors after sacrifice of mice. h Tumor mass (mg) measurement after sacrifice of mice (p-values from comparing sg1 and sg2 against LacZ control)