Fig. 3

Mining of surface proteins. a Heatmap of 120 enriched cell surface proteins. b Cell surface protein mining strategy, where the 120 proteins were first filtered for proteins with limited expression in other cancer cell lines and antibody availability. The remaining 15 proteins were then ranked based on normal tissue expression by IHC protein detection [40], median protein expression [18, 44], and median RNA expression in normal tissue datasets. The ranks across all four normal tissue datasets were summed and the highest-ranking protein, PLA2R1, was selected for further evaluation