Fig. 2

mTORC2 downregulates de novo DNA methyltransferase DNMT3A. A RNA-sequencing-based transcript expression of DNA methylating and demethylating enzymes in U87-EGFRvIII cells with siRNA against Scramble sequence or Rictor. Bar graph showed the expression level of de novo DNA methyltransferases including DNMT3A and DNMT3B in U87-EGFRvIII cells with siScramble or siRictor. KD, knockdown; ND, not detected; NS, not significant; RPKM, reads per kilobase per million mapped reads. B Transcript expression of DNMT3A gene in various types of cancers, based on TCGA datasets. GBM is highlighted in a red box. C Relative protein expression of DNMT3A in U87-EGFRvIII cells treated with drugs targeting mTORC2 substrates including Akt (Akti-1/2: 2.5 µM), SGK1 (GSK650394: 2.0 µM) and PKC-α (Bis-I: 10 µM) for 48 h