Fig. 3

β1 integrin inhibition triggers loss of oligodendroglial cells specifically in WM. Frozen spinal cord sections taken from mice exposed to normoxia or hypoxia (8% O₂) that had received daily intraperitoneal injections of the anti-mouse β1 integrin function-blocking antibody or isotype control antibody for 4 days were stained for CC1 (top two rows)) or PDGFRα (lower two rows) (A) or TER-119 (AlexaFluor-488) and CC1 (Cy-3) (D). Scale bars = 50 μm (A) or 100 μm (D). Quantification of the number of CC1 ⁺ cells/FOV (B) or PDGFRα ⁺ cells/FOV (C) after 0- or 4-days hypoxia. Results are expressed as the mean ± SEM (n = 6 mice/group). * p < 0.05. Note that β1 integrin inhibition decreased the cell density of mature oligodendrocytes and OPCs in the hypoxic spinal cord, specifically in the WM. Also note that vascular disruption led to obvious loss of oligodendrocytes in the immediate vicinity of erythrocyte deposition (D)