Astrocytic accumulation of tau fibrils isolated from Alzheimer’s disease brains induces inflammation, cell-to-cell propagation and neuronal impairment

Eltom, Khalid; Mothes, Tobias; Libard, Sylwia; Ingelsson, Martin; Erlandsson, Anna

doi:10.1186/s40478-024-01745-8

Fig. 5

From: Astrocytic accumulation of tau fibrils isolated from Alzheimer’s disease brains induces inflammation, cell-to-cell propagation and neuronal impairment

Human brain-derived tau fibrils induce a reactive state in exposed astrocytes. (a) Representative images of vimentin staining of astrocytes exposed to parietal and temporal tau fibrils. Quantification of vimentin IntDen in astrocytes exposed to (b) parietal fibrils and (c) temporal fibrils. (d) Representative images of glial fibrillary acidic protein (GFAP) staining of astrocytes. Quantification of GFAP IntDen in astrocytes exposed to (e) parietal fibrils and (f) temporal fibrils. Both for the vimentin and GFAP analysis, three AD and two control samples were used. N = 3 culture experiments, n = 10–15 non-overlapping fields per brain sample. In b, c, e and f, IntDen/image was normalized to number of nuclei. The level of significance was set at *p ≤ 0.05, **p ≤ 0.001, ***p ≤ 0.0001, ****p ≤ 0.00001. Scale bars = 100 μm

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Fig. 5

Acta Neuropathologica Communications

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