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Fig. 1 | Acta Neuropathologica Communications

Fig. 1

From: Astrocytic accumulation of tau fibrils isolated from Alzheimer’s disease brains induces inflammation, cell-to-cell propagation and neuronal impairment

Fig. 1

Extraction and characterization of human brain-derived tau fibrils. (a) Brain tissue samples were dissected from the parietal and temporal lobes of three AD patients and two controls. Extracted tau fibrils were sonicated to smaller fragments to facilitate cellular uptake. (b) Transmission electron microscopy (TEM) images confirmed the presence of tau fibrils in both AD and control brain tissue extracts. Western blot analysis of (c) total tau, (d) phosphorylated tau Ser202/Thr205, (e) 4R-tau and (f) 3R-tau concentrations in parietal (P) and temporal (T) samples confirmed their identity as tau fibrils. (g) Phospho-tau:total tau ratio and (h) 4R:3R tau ratio were calculated for each sample. Signal intensities in (c), (d), (e) and (f) were normalized to total protein concentration in each sample (Online Resource 6)

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Acta Neuropathologica Communications

ISSN: 2051-5960

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