Fig. 1

Expression of VAPB or PTPIP51 rescues mutant TDP43 induced disruption to the IP3 receptor-VDAC1 interaction. (a and b) Representative images of IP3 receptor type1-VDAC1 PLAs in SH-SY5Y cells transfected with EGFP + control (CTRL) vector, EGFP-tagged-TDP43-Q331K or -TDP43-A382T + control vector and EGFP-tagged TDP43-Q331K/EGFP-TDP43-A382T + either VAPB or PTPIP51 as indicated. TDP43 was identified via the EGFP tag and cells immunostained for β-tubulin III (artificially shown in cyan). Scale bars = 10 μm. Bar charts show PLA signals per cell after normalisation to control EGFP + CTRL cell data. Data were analysed by one-way ANOVA with Tukey’s post hoc test; (a) N = 43–63 cells from 3 independent experiments, (b) N = 38–75 cells from 3 independent experiments. Error bars are SEM; * p≤0.05, *** p≤0.001, ns not significant