Fig. 2

a Nf1^OPG mice undergo optic nerve crush (ON-CR) at 6 weeks of age, while optic nerves are analyzed at 12 weeks of age. Nf1^OPG mice following ON-CR have increased b optic nerve volumes and exhibit increased c proliferation (%Ki67⁺ cells; n = 5), d %Olig2⁺ cells (n = 7) and e %Blbp⁺ cells (n = 5). f 12-week-old Nf1^f/R1809C; hGFAP-Cre mice following optic nerve crush (ON-CR) at 6 weeks of age exhibit increased g optic nerve volumes (n = 5), h proliferation (%Ki67⁺ cells, n = 5), i %Olig2⁺ cells (n = 7) and j %Blbp⁺ cells (n = 5) compared to those undergoing a sham operation (n = 4). Data are presented as the means ± SEM. Scale bars: b, g 100 µm; c, d, e, h, i, j 50 µm. Two-tailed Student’s t test