Fig. 4

Reduction of total number of NPY⁺ neurons in the INF of MD is not associated with PR. A NPY⁺ and PR/NPY⁺ neurons (red arrows) in the INF of a control subject and an individual with MD. Higher magnification of the areas framed in a, c, e and g are shown in b, d, f and h, respectively. B Analyses between CTR and MD subjects, and their subsets on: total neurons and volume of NPY (a-d), PR/NPY (e-h), total neurons of sNPY (i-j) and the proportion of PR/NPY neurons in NPY neurons (k-l). Note the sharp drop of NPY expression in MDE compared to CE (b, j). Scale bars: a, c, e and g, 1 mm; b, d, f and h, 100 μm. Abbreviations: PR/NPY, neurons co-labeling progesterone receptor and neuropeptide Y; sNPY, neuropeptide Y neurons that did not express progesterone receptor. Note: * indicates 0.01 ≤ P<0.05, ** indicates 0.001 ≤ P<0.01, *** indicates 0.0001 ≤ P<0.001, **** indicates 0.00001 ≤ P<0.0001, Global-P<0.05