Fig. 2

Characterization of progesterone receptor immunoreactive cells in the PVN and INF. A and B Absent co-localization of PR in astrocytes (GFAP) or microglia (iba1). C and D Co-localization of PR in neural progenitors (GFAP-δ and nestin). E a PVN in the human hypothalamus. b-h Absent expression of PR in CRH⁺, TRH⁺, OXT⁺, AVP⁺, SOM⁺, TH⁺ or DYN⁺ neurons in the PVN. i Co-localization of PR in KISS1⁺ neurons in the PVN. F a INF in the human hypothalamus. b-f PR expression in POMC⁺, NPY⁺, SOM⁺, KISS1⁺ and DYN⁺ neurons in the INF. g Absent co-localization of PR in CRH⁺ neurons in the INF. Scale bars: A and D, 50 μm; B and C, 20 μm; E, a-e 30 μm, f and g 15 μm; F, a and b 20 μm, c-e 30 μm. Abbreviations: AVP, arginine-vasopressin; CRH, corticotropin-releasing hormone; DYN, dynorphin; GFAP, glial fibrillary acidic protein; GFAP-δ, glial fibrillary acidic protein-delta; Iba1, ionized calcium binding adaptor molecule 1; KISS1, kisspeptin; NPY, neuropeptide Y; OXT, oxytocin; POMC, pro-opiomelanocortin; SOM, somatostatin; TH, tyrosine hydroxylase; TRH, thyrotropin-releasing hormone (for other abbreviations see legend Fig. 1)