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Fig. 5 | Acta Neuropathologica Communications

Fig. 5

From: Interplay between androgen and CXCR4 chemokine signaling in myelin repair

Fig. 5

Inhibition of CXCR4 blocks T-dependent recruitment and differentiation of oligodendroglial cells. a and b Double labeling of GFAP (astrocytes) and eGFP (oligodendroglial cells) on spinal cord cross sections of castrated Plp-eGFP male mice at 30 dpl. Cell nuclei were counterstained in blue with DAPI. Within the LPC lesion, delimited by the dotted line, both GFAP and eGFP staining were absent in males receiving an empty implant (LPC), but in males treated with T, (LPC + T) GFAP+ astrocytic processes crossed the lesion, and eGFP+ oligodendroglial cells were aligned along them. AMD3100 blocked the T-dependent presence of GFAP immunostaining and eGFP+ cells inside the lesion. c T also induced the presence of GFAP immunostaining and eGFP+ cells within the LPC lesion of ovariectomized females, and its effect was inhibited by AMD3100. d–f Immunostaining of Olig2 (oligodendroglial cells) and CC1 (mature oligodendrocytes) on spinal cord cross sections of castrated male mice at 30 dpl. Magnification of merged panels show colocalization of Olig2 and CC1. Within the LPC lesion, delimited by the dotted line, both Olig2+ and CC1+ cells were scarce in males treated with LPC and abundant in males treated with LPC + T. The T-dependent recruitment of Olig2+ and CC1+ cells within the lesion was blocked by AMD3100. g Most of the Olig2+ cells were mature oligodendrocytes coexpressing CC1 at 30 dpl. Data are presented as means ± S.E.M. (one-way ANOVA with Tukey's multiple comparisons tests). Asterisks mark significant differences. ***P < 0.001, **P < 0.01. Scale bar: 50 µm in d merge and 20 µm in d magnification

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