Fig. 1

Remyelination, astrocyte replenishment, upregulation of CXCR4 mRNA and downregulation of MPZ mRNA by T in microdissected LPC lesions. a and b Immunostaining of MBP (a) and GFAP (b) in the LPC demyelinated lesion at 5, 10, 15 and 30 days post-lesion (dpl). Adult castrated male mice received a subcutaneous (s.c.) Silastic implant, empty (CTL, control) or filled with testosterone (T). CTL indicates LPC and CTL + T indicates LPC + T. Data are presented as means ± S.E.M. (two-way ANOVA with Tukey's multiple comparisons tests). c Positive correlation between MBP and GFAP staining within the LPC lesion (Pearson's r = 0.54, ***p < 0.001). d LPC-induced demyelination and laser microdissection of the lesion area on spinal cord sections (red square) followed by Affymetrix ChIP analysis. e and f Relative expression of two candidate genes, CXCR4 (e) and MPZ (f), assessed by qRT-PCR on RNA from LPC-demyelinated area at 5 dpl. Castrated males were treated with an empty (LPC) or T-filled (LPC + T) implant. Analysis of CXCR4 (g) and MPZ (h) in uninjured control animals (treated with PBS instead of LPC). Data are presented as means ± S.E.M. (two-tailed unpaired Student's t-test). i Immunostaining of CXCR4 in the LPC demyelinated lesion at 5, 10, 15 and 30 dpl. Castrated males were treated with an empty (LPC) or T-filled (LPC + T) implant. Data are presented as means ± S.E.M. (two-way ANOVA with Tukey's multiple comparisons tests). j Relative expression of CXCR4, assessed by qRT-PCR on RNA extracted from the spinal cord of AR^Lox/Lox and AR.^NesCre male mice. Data are presented as means ± S.E.M. (two-tailed unpaired Student's t-test). Asterisks mark significant differences. ***P < 0.001, **P < 0.01, *P < 0.05