Fig. 5

Deficiency in microtubule-associated motor proteins exacerbates TDP-43 pathology in Drosophila. a Light microscopic images of the compound eyes of EGFP flies or TDP-43 flies coexpressing EGFP-IR or inverted repeat RNAs against DCTN2 (p50, dynamitin), dynein heavy chain (Dhc64C), dynein light chain (Dlc), kinesin heavy chain (Khc), or kinesin light chain (Klc), under the control of the GMR-Gal4 driver. TDP-43 flies coexpressing DCTN2-IR#2 were not analyzed due to their lethality at the pupal stage. b Bar graph showing the area of remaining eye pigment of TDP-43 flies in a. Each dot represents data from each fly (n = 8–26 flies). c Confocal microscopic images of TDP-43 antibody staining of larval eye discs of TDP-43 flies coexpressing EGFP-IR, dDCTN2-IR#1, Dlc-IR#2, or Klc-IR#1. Arrows indicate cytoplasmic inclusions of TDP-43. Hoechst 33342 was used for nuclear staining. Scale bars, 20 µm (left) and 10 μm (right). d Bar graph showing the ratio of cells with cytoplasmic inclusions of TDP-43 in (c). Data are presented as the mean ± SEM of 7–9 fly larvae. Statistical analyses in b and d were performed to assess differences from the EGFP-IR-expressing TDP-43 flies by one-way ANOVA followed by the Dunnett multiple comparison test (**p < 0.01, ***p < 0.001, ****p < 0.0001)