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Fig. 2 | Acta Neuropathologica Communications

Fig. 2

From: Blood-based CNS regionally and neuronally enriched extracellular vesicles carrying pTau217 for Alzheimer’s disease diagnosis and differential diagnosis

Fig. 2

Characterization of EVs enriched by ultracentrifugation a EVs structure revealed by cryo-EM showed double layered membrane-bound vesicles with a diameter ≈100 nm. b NTA showed a population of EVs with a peak ≈100 nm (n = 3). Three lines with different colors represent three replicate experiments. c EVs and neuron marker proteins were present in the EVs fraction obtained by ultracentrifugation; meanwhile, the non-EVs proteins, albumin was absented in the EVs fraction. d Graph summarized the relative content of EVs/neuron marker proteins and non-EVs proteins of WB experiments (n = 3). e STORM imaging of neuron marker GABRD (green) with EVs marker CD9 (red) and pTau217 (violet) presence of EVs membranes together. Scale bar = 0.1 μm. f STORM imaging was performed to confirm GPR162 (green), CD9 (red) and pTau217 (violet) presence of EVs membranes together. Overlap of both markers with CD9 indicates their presence on EVs membranes. Scale bar = 0.1, μm. **p < 0.01, ***p < 0.001, ****p < 0.0001, one-tailed nonparametric ANOVA followed by Kruskal–Wallis test

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