Fig. 7

Down-regulation of Gnao1 expression in SCs accelerated SC differentiation. A Establishment of SC in vitro differentiation model. Immunocytochemical analyses of MAG and EdU label assay showed that MAG expression (green) increased and proliferation ability (green) decreased of 3 day-induction SCs with db-cAMP (a1), which was further proved by WB analysis (a2) and qPCR (a3), indicating that the in vitro differentiation model was successfully established. Scale bar in (a1) = 100 μm. T-test, n = 3, Ns, p > 0.05 vs controls, no statistical difference. *, p < 0.05, **, p < 0.01, and ***, p < 0.001 vs controls. B Gnao1 deletion in SCs accelerated SC differentiation. Bb1 Representative immunocytochemical analyses of MAG (green) and Gnao1 (red) showed that the differentiation of SCs treated with Gnao1-shRNA or Scramble, indicating that knockdown Gnao1of SCs promote SC differentiation. Scale bar = 100 μm. Bb2 WB comparing MAG and P0 levels in differentiated cells treated with Gnao1-shRNA or Scramble. Also shown are representative WB images, with GAPDH as the internal standard. T-test, n = 3, ***, p < 0.001 vs scramble. Bb3 QPCR comparing mRNA levels of MAG, P0, MBP, PMP22 and Gnao1 in differentiated SCs treated with Gnao1-shRNA or Scramble. T-test, n = 3, *, p < 0.05, **, p < 0.01 and ***, p < 0.001 vs Scramble