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Fig. 6 | Acta Neuropathologica Communications

Fig. 6

From: The Schwann cell-specific G-protein Gαo (Gnao1) is a cell-intrinsic controller contributing to the regulation of myelination in peripheral nerve system

Fig. 6

Down-regulation of Gnao1 expression in SCs reduced migration of SCs seeded on PDL (scratch migration assay), but resulted in increased migration when SCs are seeded on fasciculated DRG axons. A Results of an insert-based scratch migration assay. The left diagram shows the experimental process, and the right shows SCs transfected with Gnao1-shRNA reduced migration compared with that of SCs transfected with Scramble-shRNA. scale bar = 500 μm. The red dashed circle indicates the area of the scratch that has not healed. T-test, n = 4, Ns, p > 0.05 vs scramble, no statistical difference. ***, p < 0.001 vs scramble. B Results of a cell aggregation migration assay on the fasciculated DRG axons. The left diagram shows the experimental process, and the right shows that Gnao1 knockdown in SCs significantly increases SC migration from the SC aggregates on fasciculated DRG axons. Scale bar = 500 μm. Red dashed circles indicate the area of SC migration. T-test, n = 7, **, p < 0.01 vs Scramble

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Acta Neuropathologica Communications

ISSN: 2051-5960

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