Fig. 1

Screening of novel myelination-related G proteins and GPCRs and hypothesis of their regulation of myelination. A Heat map of the changes in the expression of G proteins and GPCRs during SC myelination (columns). The red dot box indicates 3 genes (GPR149, GPR37 and Gnao1) with the same expression trend. B QPCR analysis of the expression dynamics of 3 genes (GPR149, GPR37 and Gnao1) in SCs during myelination. n = 3. C The schematic diagram showing the possible mechanisms of Gnao1 in regulating SC myelination inferred from data analysis and co-expression assays, that is, on the initial stage of myelination (i.e., the preparation stage), the up-regulation of Gnao1 expression (red up arrow) may promote immature SC proliferation and migration; while the subsequent down-regulation of Gnao1 (green down arrow) may be related to SC differentiation, indicating entry into the pre-myelination stage. With the completion of SC differentiation, the expression of Gnao1 rose again, at which time the mature SCs began to wrap the axons and form myelin sheaths