Fig. 2

rTBI causes extensive neuronal loss at 1 month after injury that is attenuated by genetic ablation but not haploinsufficiency of Sarm1. (A) Loss of NeuN stained neurons in the cerebral cortex of Sarm1^+/+ and Sarm1^+/− mice after rTBI (images were taken from an area approximately corresponding to the filled square in the inset; rectangles indicate the approximate region of interest (ROI) used for quantitative analyses shown in panel [B]). (B) Although Sarm1^+/− mice had numerically more neurons in the impacted hemisphere than Sarm1^+/+ mice, this did not reach statistical significance. In contrast, genetic ablation of Sarm1 significantly suppressed neuronal loss (p = 0.028 for group effect, p < 0.001 for ROI, p = 0.010 for group x ROI interaction). Data in the bar graph are shown as mean ± sem. n = 9 per group. *p < 0.05. **p < 0.01. Scale bar = 50 μm