Fig. 7

Immunofluorescence confocal image show Rab10 and pRab10 expression at the synapse in the C57BL/6J WT mouse brain. Triple labeling was performed (N = 3): Rab10/VAMP2/Homer or pRab10/VAMP2/Homer, with secondary antibodies anti-rabbit Alexa 488, anti-mouse Alexa 555, and anti-chicken Alexa 647. Here the Homer1 channel color was artificially changed to blue for all images. a Rab10 (green) colocalized with the presynaptic marker VAMP2 (red) and with the post synaptic marker Homer1 (blue), indicated by arrow, shown in merged and zoomed in image Scale bar = 10, 2 µm. b Distance between Rab10 and Vamp2 (0.0373 µm) and distance between Rab10 and Homer1 (0.0236 µm). c pRab10 (green) colocalized with the presynaptic marker VAMP2 (red), indicated by arrow but did not colocalize with the post synaptic marker Homer1 (blue), indicated by arrow, shown in merged and zoomed in image. Scale bar = 10, 2 µm. d Distance between pRab10 and Vamp2 (0.0353 µm) and between pRab10 and Homer1 (0.288 µm). e Rab10 (green) colocalized with α-synuclein (red) shown in merged and zoomed in image. Scale bar = 10, 2 µm. f pRab10 (green) colocalized with α synuclein (red) shown in merged and zoomed in image. Scale bar = 10, 2 µm. g Distance between Rab10 and α-synuclein (0.0361) and distance between pRab10 and α synuclein (0.0347 µm). (n = 3 biologically independent mouse brain samples). h In human brain temporal cortex, pRab10 (green) colocalized with the presynaptic marker VAMP2 (red), indicated by arrow but did not colocalized with the post synaptic marker Homer1 (blue), indicated by arrow shown in merged and zoomed in image. Scale bar = 10, 2 µm. i Distance between pRab10 and VAMP2 (0.0283 µm) and between pRab10 and Homer1 (0.383 µm). (n = 3 human brain samples)