Fig. 6

Immunofluorescence confocal images showing Rab10 and pRab10 expression in subcellular compartments in the C57BL/6J WT mouse cortex. a Rab10 (green) colocalized with the endoplasmic reticulum (ER) marker, KDEL (red) shown in merged and zoomed in images. Scale bar = 10, 2 µm. b pRab10 (green) did not show colocalization with the ER marker, KDEL (red) shown in merged and zoomed in image. Scale bar = 10, 2 µm. c Colocalization analyses using Mander’s colocalization coefficient (MCC) indicated a significant difference Rab10 protein colocalization with KDEL compared to pRab10 (p value = 0.001). d Rab10 (green) colocalized with the trans Golgi network (TGN) marker, TGN46 (red) shown in merged and zoomed in image. Scale bar = 10, 2 µm. e pRab10 (green) did not show colocalization with TGN46 (red) shown in merged and zoomed in image. Scale bar = 10, 2 µm. f Colocalization analyses using MCC indicated a significant difference in Rab10 protein colocalization with TGN46 compared to pRab10/TGN46 (p value = 0.01). g Rab10 (green) colocalized with the lysosomal marker, LAMP1 (red) shown in merged and zoomed in image. Scale bar = 10, 2 µm. h pRab10 (green) did not show colocalization with LAMP1 shown in merged and zoomed in image. Scale bar = 10, 2 µm. i Colocalization analyses using MCC indicated a significant difference in Rab10 protein colocalization with LAMP1 compared to pRab10 (p value = 0.004) j Rab10 (green) did not show colocalization with the early endosome marker, EEA1 (red) shown in merged and zoomed in image. Scale bar = 10, 2 µm. k pRab10 (green) did not show colocalization with EEA1 shown in merged and zoomed in image Scale bar = 10, 2 µm. l There was no significant difference between Rab10 and pRab10 colocalization signal with EEA1 indicated by MCC (p value = 0.4). Colocalization analysis was performed using ImageJ plug in, JACoP, and unpaired t-test with Welch’s correction was used to get the significance value. (n = 3 biologically independent mouse brain samples)