Fig. 5

Rab10 and pRab10 immunofluorescence in Glia cell types. Confocal laser scanning microscopy images of C57BL/6J WT mouse brain sections from cortex. a A representative confocal image shows Rab10 (green) expression in CD68 (red) positive microglia cells shown in both the merged lower and higher magnification images indicated by arrow. Scale bar = 50, 5 µm. b A representative confocal image shows pRab10 (green) expression in CD68 (red) positive cells shown in both the merged lower and higher magnification images indicated by arrow. Hoechst 33,342 staining (blue) shows the cell nucleus. Scale bar = 50, 5 µm. c A representative image shows Rab10 (green) expression in GFAP expressing astrocytes (red) shown in the merged lower and higher magnification images indicated by arrows. Hoechst 33,342 staining (blue) shows the cell nucleus. Scale bar = 20, 5 µm. d. A representative image shows pRab10 (green) expression in GFAP expressing astrocytes (red) shown in merged lower and higher magnification images indicated by arrows. Hoechst 33,342 staining (blue) shows the cell nucleus. Scale bar = 20, 5 µm. e A representative image shows Rab10 (green) expression in Olig2 expressing oligodendrocyte cells (red) shown in merged lower and higher magnification images indicated by arrows. Hoechst 33,342 staining (blue) shows the cell nucleus. Scale bar = 50, 5 µm. f A representative image shows pRab10 (green) expression in Olig2 expressing oligodendrocyte cells (red) shown in merged lower and higher magnification images indicated by arrows. Hoechst 33,342 staining (blue) shows the cell nucleus. Scale bar = 50, 5 µm. (n = 3 biologically independent mouse brain samples)