Fig. 4

Rab10 and pRab10 immunofluorescence and confocal microscopy images in C57BL/6J WT mouse SNc sections. a Low magnification confocal image shows Rab10 (green) is expressed in the SNc brain area, surrounded by a box. DAT (red) and TH (blue) immunofluorescence highlights the SNc brain and merged channel image shows Rab10 expression in this region. Scale bar = 500 µm. b Higher magnification image shows Rab10 (green) enrichment in DAT (red) and TH (blue) immuno-positive cells in SNc, indicated by arrows Scale bar = 50 µm. c Low magnification confocal image shows pRab10 (green) is expressed in the SNc. DAT (red) and TH (blue) immunofluorescence to highlight the SNc and merged channel image shows pRab10 expression in this region Scale bar = 500 µm. d Higher magnification image shows pRab10 (green) enrichment in DAT (red) and TH (blue) immuno-positive cells in the SNc indicated by arrows. Scale bar = 50 µm. (n = 3 biologically independent mouse brain samples)