Fig. 2

Localization of Rab10 and pRab10 in excitatory neurons and interneurons the cortex. C57BL/6JWT mice at 3–4 months age were used for immunofluorescence localization in the cortex. a Rab10 (green) was present in all cortex layers. SATB2, a marker for excitatory neurons, was visualized in red. The merged image showed Rab10 (green) was localized in SATB2 positive excitatory neurons expressing neurons. Scale bar = 50 µm. b Rab10 (green) was localized in SATB2 (red) expressing excitatory neurons. Scale bar = 50 µm. c pRab10 (green) was present in all cortex layers. SATB2 (red) was expressed in expressed in all cortex layers except layer I and merged image shows pRab10 (green) is localized in SATB2 (red) expressing neurons. Scale bar = 50 µm. d High magnification image showed that pRab10 (green) was enriched in SATB2 (red) expressing excitatory neurons. Scale bar = 50 µm. e A representative high magnification image showed Rab10 is expressed in calretinin expressing inhibitory neurons indicated by arrows. Scale bar = 50 µm. f A representative high magnification image shows pRab10 was expressed in calretinin expressing inhibitory neurons indicated by arrows. Scale bar = 50 µm. (n = 3 biologically independent mouse brain samples)