Fig. 5

FUS-16R impaired the homeostatic movement of PSD-95 within the synapse. A Representative time course images of endogenous PSD95 puncta prior to and post photobleaching (arrow) for Control, FUS-WT, FUS-16R, FUS-16R-NLS, FUS-16R-LLPS transfected neurons and control neurons preincubated in anisomycin. B, C Average normalised fluorescence intensity recovery of the photobleached endogenous PSD95 puncta over time and at 5 s prior to the final time point for Control (white circles; n = 14), FUS-WT (grey circles; n = 13), FUS-16R (green circles; n = 13) transfected neurons and Anisomycin-treated neurons (orange circle; n = 14). D, E Average normalised fluorescence intensity recovery of the photobleached endogenous PSD95 puncta over time and at 5 s prior to the final time point for FUS-WT (grey circles; n = 13), FUS-16R (green circles; n = 13), FUS-16R-NLS (cyan circles; n = 15) and FUS-16R-LLPS (blue circles; n = 12) (F). *p < 0.05, ** p < 0.01, **** p < 0.0001, post hoc Tukey analysis