Fig. 4

FUS-16R induced synapse dysfunction, inhibiting the initiation of single spine structural plasticity. A–E Representative time course images of dendritic spines prior to and following single spine glutamate uncaging for stimulated spines (top) and unstimulated spines (bottom) for neurons transfected with FUS-16R (A), Venus (structural marker control) (B), FUS-WT (C), and FUS-16R-NLS (D) and FUS-16R-LLPS (E). The corresponding time course graph illustrating the average change in spine head areas, as a percentage of baseline, and histogram illustrating the final (12-min post stimulation) change in area for FUS-16R (a; p = 0.405, n = 9), Venus (b; p = 0.003, n = 8), FUS-WT (c; p = 0.008, n = 6,), and FUS-16R-NLS (d; p = 0.001, n = 8,) and FUS-16R-LLPS (e; p = .0001, n = 6). ** p < 0.01, ***p < 0.001, unpaired t-test