Fig. 3
FUS-16R induced a reduction of AMPA- and NMDA-R evoked EPSCs in a manner which is dependent on its localisation and ability to undergo liquid–liquid phase separation. A, D, G, J Representative widefield images illustrating the FUS expression and localisation for FUS-WT (A), FUS-16R (D), FUS-16R-NLS (G) and FUS-16R-LLPS (J) transfected neurons. B, C, E, F, H, I, K, L Pair-wise analysis of the amplitude of basal EPSCs (EPSCAMPA and EPSCNMDA) between untransfected and neighbouring transfected neurons for FUS-WT (B, C), FUS-16R (E, F), FUS-16R-NLS (H, I) and FUS-16R-LLPS (K, L). Representative single trances of EPSCAMPA and EPSCNMDA recorded at − 70 and + 40 mV holding current respectively, and evoked via Schaffer Collateral stimulation, black arrow indicates peak amplitude measurement. P < 0.05, unpaired t-test