Fig. 2

All FUS-16R constructs, except FUS-16R-LLPS, can form aggregates, and only FUS-16R induced a significant reduction in the PSD95 puncta density and area. A Representative images of FUS-WT, FUS-16R, FUS-16R-NLS and FUS-16R-LLPS transfected HEK cells, illustrating the ability of all constructs except FUS-16R-LLPS to form FUS aggregates. B Representative images of CA1 neurons expressing either FUS-WT, FUS-16R-EYFP (FUS-16R), FUS-16R-NLS or FUS-16R-LLPS. C Representative images of endogenous PSD95 puncta (green) in control, FUS-WT, FUS-16R, FUS-16R-NLS and FUS-16R-LLPS transfected neurons. D, E Analysis of PSD95 puncta number (D) and puncta area (E) for control (n = 15), FUS-WT (n = 14), FUS-16R (n = 17), FUS-16R-NLS (n = 17) and FUS-16R-LLPS (n = 12) transfected neurons within 20 µm-sized ROIs. * p < 0.05, ** p < 0.01, **** p < 0.0001, post hoc Tukey analysis (One way ANOVA)