Fig. 6
Post-reperfusion treatment with intravenous recombinant human MANF decreases infarct volume. a Timeline of the experiment. The black arrow indicates intravenous administration of rhMANF or vehicle. RhMANF (1.5 µg or saline was administered to the rats 15 min after reperfusion. dMCAo = distal middle cerebral artery occlusion, TTC = 2,3,5-triphenyltetrazolium chloride staining. b Infarction volume was determined 2 days after dMCAo by TTC staining, n = 16 per group, (**p < 0.01), Student’s t-test. c Distribution of infarction along the rostrocaudal axis.d Representative images of TTC stained brain sections from vehicle and rhMANF groups. The values are expressed as mean ± SD