Fig. 6
DX2 prevents behavioural deficits in the 6-OHDA -induced PD model A Scheme of AAV-DX2 transduction in the 6-OHDA-induced mouse model. B AAV-DX2-treated mice showed lower levels of contralateral rotation compared to saline or vehicle (GFP), indicating that DX2 attenuated damage in dopaminergic neurons. C DX2-treated mice showed increased contralateral forepaw contacts, indicating that AAV-DX2 attenuated unilateral damage in dopaminergic neurons. D AAV-DX2 treated mice show less right-biased body swing. Animals: saline (saline-treated wild type mice), n = 4; GFP (GFP-injected 6-OHDA-treated mice), n = 5; DX2 (DX2-injected 6-OHDA-treated mice), n = 11. AAV: AAV-GFP, 4 × 109 vg; AAV-DX2, 4 × 109 vg. E Immunofluorescence image of the GFP and DX2-injected mouse brain. The white square box indicates TH-positive dopaminergic neuronal cells and the yellow arrows show the virus injection site. The relative fluoresence. F Relative fluoresence between AAV-GFP and AAV-DX2 group. Quantification of fluoresence intensity was conducted using the Image J software. L: Left. R: Right. G Immunoblot assay of the GFP and DX2 injected mice. The cleaved PARP-1 specific antibody was used. Which lanes were randomly selected mice in each group. H Immunoblot assay about the samples from AAV-GFP or DX2-injected mice under 6-OHDA treated comdition. The parylation-specific antibody was used. Which lanes were randomly selected mice in each group. I and J DX2 and Bax mRNA expression of naïve, 6-OHDA, and DX2-treated mice. ns Non-significant; *P < 0.05; **P < 0.01; ***P < 0.001