Fig. 4
AAV-DX2 has anti-apoptotic effects and alters cellular signalling. A Cytotoxic effects in the primary neurons (Neuron), MEF (Mouse embryonic fibroblasts), hepatocytes, and MSC (Mesenchymal stem cells). After H2O2 (200 μM, 24 h) treatment, a decreased cytotoxic effect was observed in DX2 transduced cells (DX2) when compared with control-transduced cells. The measurement of cell death was conducted through cell counting. B DX2 is not required for normal cell growth in SH-SY5Y (left) and primary neuronal cells (right). In oxidative stress conditions, DX2-infected cells (AAV-DX2, DX2) showed decreased levels of cell death when compared to their control counterparts (AAV-GFP, GFP). The measurement of cell death was again performed using cell counting. C Graph of gene counts representing number of survival-related and death-related genes in each pathway. D P-value plot of the signalling pathway changed by DX2 overexpression E Enrichment plot of RNAseq of AAV-GFP or AAV DX2-infected neuroblastoma cell. F Graph of gene counts representing cell death and inflammatory related pathways were downregulated.; ns Non-significant; *P < 0.05; **P < 0.01; ****P < 0.0001 (t-test)