Fig. 1

APPDup and NDC iPSC lines differentiate successfully into excitatory cortical neurons. A Representative immunofluorescence staining for DAPI, beta tubulin III, NeuN and synapsin1 in APPDup and NDC neurons day 21 CTRL RNAi-treated neurons. B No significant differences in quantification NeuN + % nuclei were observed among APPDup and NDC differentiated neurons (p = 0.814, one-way ANOVA). Error bars indicate s.e.m. C Principal Component Analysis (PCA) plot of variation of top RNA-Seq genes from differentiated neurons and iPSCs from all cell lines. D Volcano plot depicting differentially expressed genes (DEGs) between APPDup and NDC CTRL RNAi-treated day 21 neurons and iPSCs. Significance and foldchange threshold of p-adj. < 0.005 and log2FC > 0.75 were used. Max -log10(p) value plotted = 300. E Heatmap representing selected neuronal, iPSC, and glial marker gene expression in APPDup and NDC day 21 neurons and iPSCs