Fig. 2

Non-NP are transformed into NP in the progression of ADNC using p-tau staining as a NP marker in the frontal cortex. a Low magnification (300 μm) and high magnification (60 μm) images of frontal cortex in different AD neuropathological changes. Non-NP, NP, and total plaque count were compared between low AD (n = 18), intermediate AD (n = 17), high AD (n = 23), and high AD mixed pathology (n = 21). NP and non-NP were determined by using p-tau (7F2) staining and anti-Aβ antibody D12B2 (anti-Rabbit). b Comparing intermediate AD and high AD, the non-NP count is not changed, but the NP count is significantly higher in high AD cases. The manual count of total plaques shares a similar trend with the software (Qupath) quantification of the percent positivity of amyloid plaque from consecutive tissues