Fig. 5

miR-211 is a metabolic regulator in MB cells. A Global metabolic network analysis of differentially abundant metabolites in MB expressing miR-211 or vector only (V/O) cells. FDR-corrected p-value shown. B Quantitative analysis of intermediates for essential amino acid (EAA) and glutamine metabolism in D425 cells expressing miR-211 compared with control. C Oxygen consumption rate (OCR) was analyzed using the Seahorse XF analyzer in D425 vector only (V/O) or miR-211 overexpressing cells. D Basal OCR were measured at three time points, followed by sequential injections of the ATP synthase inhibitor oligomycin, the uncoupler FCCP, the complex I inhibitor rotenone, and the complex III inhibitor antimycin A. E The effect of miR-211 overexpression on the mitochondrial content of human D425 cells by MitoTracker Red staining. Red, MitoTracker; Blue, Hoechst 33,258. Scale bars, 10 μm. (F) Ratio of mitochondrial gene ND1 and lipoprotein lipase (LPL) to genomic β-actin in D425 vector only (V/O) or miR-211 overexpressing cells. G Caspase 3/7 activities were detected in D425 vector only (V/O) or miR-211 overexpressing cells. Data, mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, NS, non-significant