Fig. 4

miR-211 inhibits MB progression by targeting ACSL4 expression. A Volcano plot of differentially expressed genes in MB expressing miR-211 or vector only (V/O) cells. B Venn diagram revealing the overlap of potential target genes based on miR-211 targets identified in the TargetScan database and the RNA-seq data of downregulated genes from MB cells. C Expression levels of potential miR-211 targets ACSL4, RAB22A, and SERINC3 in MB cells expressing vector only (V/O) or miR-211 were validated by qRT-PCR. D Relative protein levels of ACSL4, RAB22A, and SERINC3 were detected by western blotting after miR-211 overexpression in MB cells. E Luciferase activity assay for targeting sequences of the wild or mutant ACSL4 3′-UTR by miR-211 in D425 cells. F D425 cells expressing miR-211 or vector only (V/O) were transiently transfected with ACSL4 plasmid, and lysates were subjected to qRT-PCR and western blotting. G Cell viability was determined by MTS assays in D425 cells overexpressing miR-211 with and without introduction of ACSL4 plasmid. H Cell invasive potential was determined by transwell assays in D425 cells overexpressing miR-211 with and without introduction of ACSL4 plasmid. Data, mean ± SD. ***P < 0.001, NS, non-significant