Fig. 3

miR-211 inhibits the tumorigenicity of MB cells. A MB cells expressing vector only (V/O) or miR-211 were stably transfected with a renilla luciferase expressing construct and implanted into the cerebella of NOD-SCID mice. Tumor formation was assessed weekly after tumor implantation by bioluminescent signal detection with the IVIS platform. B Quantification of total photon counts from mice implanted with MB cells expressing V/O or miR-211. C Fluorescent staining of Ki-67 and TUNEL in the D425 xenografts on day 35 after intracranial injection. Images in the lower column are the magnified images within the white boxes found in the middle column. Nuclei are stained with DAPI (blue). Scale bars, 100 μm. Data, mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001