Skip to main content

Table 3 Re-testing pyrosequencing samples with DNA methylation array and droplet digital PCR

From: Variant allelic frequencies of driver mutations can identify gliomas with potentially false-negative MGMT promoter methylation results

Patient

*Sample

Sex

Age

TERT VAF

MGMT pyroseq result

MGMT array result

MGMT ddPCR result

#1

Pyro-311

M

61

0.02

1.49% (negative)

negative

negative

#2

Pyro-293

F

55

0.02

6.44% (negative)

equivocal

positive

#3

Pyro-049

M

53

0.04

5.52% (negative)

positive

equivocal

#4

Pyro-023

F

47

0.07

3.73% (negative

negative

QNS

#5

Pyro-081

F

69

0.10

11.06% (positive)

positive

positive

#6

Pyro-182

F

69

0.10

4.85% (negative)

negative

QNS

#7

Pyro-083

F

46

0.25

2.97% (negative)

negative

negative

#8

Pyro-200

M

53

0.28

3.18% (negative)

negative

QNS

#9

Pyro-152

F

56

0.35

4.01% (negative)

negative

negative

#10

Pyro-053

F

69

0.44

35.56% (positive)

positive

positive

#11

Pyro-417

F

69

0.49

3.20% (negative)

negative

negative

#12

Pyro-243

M

61

0.78

3.03% (negative)

negative

negative

  1. * Original pyrosequencing sample ID in Supplementary Table S2; pyroseq: pyrosequencing; ddPCR: droplet digital PCR; QNS: quality/quantity of DNA not sufficient for reliable test result