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Fig. 5 | Acta Neuropathologica Communications

Fig. 5

From: Variant allelic frequencies of driver mutations can identify gliomas with potentially false-negative MGMT promoter methylation results

Fig. 5

False negative results in IDH-wildtype glioblastoma with lowTERTVAF. (A) MGMT promoter methylation results for 12 GBM samples (6 with TERT VAF ≤ 0.10, 6 with TERT VAF ≥ 0.25) comparing initial pyrosequencing methylation scores (left Y-axis, cutoff for positive = 10.0%, horizontal solid red line) to results on re-testing with DNA methylation array (right Y-axis). (B) MGMT promoter methylation results for the same 12 GBM samples comparing initial pyrosequencing methylation levels to results on re-testing with ddPCR. (GBM: IDH-wildtype glioblastoma, pos: positive, equiv: equivocal, neg: negative, QNS: quality/quantity of DNA not sufficient for reliable test result, ddPCR: droplet digital PCR)

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Acta Neuropathologica Communications

ISSN: 2051-5960

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