Fig. 9

UNC2025 and TSA treatment abrogate invasion capacity of WHO grade 2 spheroids (a) Representative images of the 3D Matrigel invasion assay showing invasion capacity of WHO grade 2 spheroids at 24 (top panel) and 48 (middle, bottom) hours following monotherapy and combination therapy of UNC2025 and TSA at various concentrations (0.5 µM; 1 µM) compared to vehicle control (0.1% DMSO, 0.1% Ethanol) (n = 3). Images were taken using bright field microscopy (top and middle panel), and confocal microscopy (bottom panel). Scale bar in each panel represents 200 µm. Cell nuclei are stained with DAPI (blue) and Phalloidin (green). b, c Bar graphs showing the quantification of relative invasion at b 24 h and c 48 h presented as max. diameter of the total invasive zone in µm. Error bars indicate standard error of mean. One-way ANOVA with Dunett’s test for multiple comparisons was used for statistical evaluation; ns = not significant, **p < 0.01, ***p < 0.001, ****p < 0.001