Fig. 3

Patient-derived spheroids preserve histology, protein expression and immune components of matched tumour tissues. Representative immunostaining images of a WHO grade 1 (n = 6) and b WHO grade 2 (n = 1) of patient tumour tissues (Tumour) and matched patient-derived meningioma spheroids (3D). Spheroids were fixed and embedded six days after seeding, corresponding to three days after spheroid formation. Stainings are shown in order: H&E, anti-SSTR2, anti-Ki67, anti-CD68 and anti-CD163. Scale bars: 200 µm. c Plot of immunoscores of stainings displayed in a and b in tissues, T, (triangle) and spheroids, S, (circle) (n = 7). Colour represents immunoscores of 0–4 (0 = negative, 1 = weak, 2 = moderate, 3 = strong, 4 = very strong) in orange for grade 1 and purple for grade 2. Grey dot indicates no scoring