Fig. 2
Patient-derived meningioma spheroid size is controlled by seeding density and remains stable over time. a Representative brightfield images of patient-derived meningioma spheroids 72 h post-seeding for seeding density of 1000, 3000 and 5000 cells. Scale bar in each panel: 200 µm (Leica IM8). b, c Bar graphs of (b) spheroid diameter in µm and (c) spheroid volume in µm3 72 h post-seeding for spheroids of 1000, 3000 and 5000 cells (n = 6). d Dot plot showing the average standard deviations of spheroid diameter (µm) of spheroids generated from the same sample. e Relative fold change in spheroid diameter from spheroid formation to 14 days post-seeding. Each line represents the mean of 5 independent experiments ± standard error for 1000 cells (blue), 3000 cells (green) and 5000 cells (grey). f Representative bright-field images of spheroids over 14 days. Scale bar in each panel: 200 µm (Leica IM8). Paired t-test was used for statistical evaluation. *p < 0.05, **p < 0.01, ***p < 0.001